H00010062-M08

antibody from Abnova Corporation

Targeting: NR1H3 LXR-a, LXRa, RLD-1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010062-M08

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010062-M08, RRID:AB_10719393

Product name

NR1H3 monoclonal antibody (M08), clone 3E9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant NR1H3.

Antigen sequence

MSLWLGAPVPDIPPDSAVELWKPGAQDASSQAQGG
SSCILREEARMPHSAGGTAGVGLEAAEPTALLTRA
EPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKA
SGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHC
PMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIR
LKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQ
LGMIEKLVAAQQQCNRRSFSDRLRVTVMLLETSRR
YNPGSESITFLKDFSYNREDFAKAGLQVEFINPIF
EFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQ
LQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMK
LVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDV
HE


Isotype

IgG

Antibody clone number

3E9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Knock-down of the oxysterol receptor LXRα impairs cholesterol efflux in human primary macrophages: lack of compensation by LXRβ activation.

Ishibashi M, Filomenko R, Rébé C, Chevriaux A, Varin A, Derangère V, Bessède G, Gambert P, Lagrost L, Masson D
Biochemical pharmacology 2013 Jul 1;86(1):122-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NR1H3 expression in transfected 293T cell line by NR1H3 monoclonal antibody (M08), clone 3E9.Lane 1: NR1H3 transfected lysate(45.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NR1H3 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol