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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A302-144A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MADD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MADD by immunohistochemistry. Sample: FFPE sections of human breast carcinoma and colon carcinoma. Antibody: Affinity purified rabbit anti-MADD (Cat. No. A302-144A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MADD by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MADD antibody A302-144A used for IP at 3 µg/mg lysate. MADD was also immunoprecipitated by rabbit anti-MADD antibody A302-143A, which recognizes an upstream epitope. For blotting immunoprecipitated MADD, A302-143A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
