PAB8161

antibody from Abnova Corporation

Targeting: GIT1

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB8161

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB8161, RRID:AB_1708084

Product name

GIT1 (phospho Y554) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of GIT1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Regulation of adaptor protein GIT1 in platelets, leading to the interaction between GIT1 and integrin alpha(IIb)beta3.

Sato H, Suzuki-Inoue K, Inoue O, Ozaki Y
Biochemical and biophysical research communications 2008 Mar 28;368(1):157-61

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Role of phospholipase Cgamma1 in cell spreading requires association with a beta-Pix/GIT1-containing complex, leading to activation of Cdc42 and Rac1.

Jones NP, Katan M
Molecular and cellular biology 2007 Aug;27(16):5790-805

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GIT1 utilizes a focal adhesion targeting-homology domain to bind paxillin.

Schmalzigaug R, Garron ML, Roseman JT, Xing Y, Davidson CE, Arold ST, Premont RT
Cellular signalling 2007 Aug;19(8):1733-44

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of GIT1 (phospho Y554) polyclonal antibody (Cat # PAB8161) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.

Validation comment

Dot Blot (Peptide)