PAB20257

antibody from Abnova Corporation

Targeting: GIT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20257

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20257, RRID:AB_10963095

Product name

GIT1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant GIT1.

Antigen sequence

HSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTP
SSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSG
DPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGL
PSTEDVILKTEQVTKNIQELLRAAQEFKHDS


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with GIT1 polyclonal antibody (Cat # PAB20257) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with GIT1 polyclonal antibody (Cat # PAB20257) at 1-4 ug/mL dilution shows positivity in cytoplasm, focal adhesions.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with GIT1 polyclonal antibody (Cat # PAB20257) shows strong cytoplasmic positivity in neuronal cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)