Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 935601 - Provider product page
- Provider
- BioLegend
- Product name
- Purified anti-GIT1
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W18253B
- Vial size
- 25 µg
- Concentration
- 0.50 mg/mL
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-GIT1 antibody (clone W18253B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. Cell lysates were loaded in order of ascending GIT1 expression; predicted expression data was obtained from Human Protein Atlas.
- Conjugate
- Unconjugated