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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA3-16515 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AIP Monoclonal Antibody (35-2)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody is specific for the FKBP domain.
- Antibody clone number
- 35-2
- Concentration
- Conc. Not Determined
Submitted references Mechanistic insights into cancer cell killing through interaction of phosphodiesterase 3A and schlafen family member 12.
Wu X, Schnitzler GR, Gao GF, Diamond B, Baker AR, Kaplan B, Williamson K, Westlake L, Lorrey S, Lewis TA, Garvie CW, Lange M, Hayat S, Seidel H, Doench J, Cherniack AD, Kopitz C, Meyerson M, Greulich H
The Journal of biological chemistry 2020 Mar 13;295(11):3431-3446
The Journal of biological chemistry 2020 Mar 13;295(11):3431-3446
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of Ara9 in mouse lung lysates (30 µg) using Product # MA3-16515 at 1:500 with a 1 second exposure.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB detection on HEK 293T/17 cells. Lane 1: IP ARA9, WB ARA9, Lane 2: WB ARA9 with whole cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AIP in 0.5 mg/mL Hek293 lysate. Samples were incubated in AIP monoclonal antibody (Product # MA3-16515). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AIP in 0.5 mg/mL Hek293 lysate. Samples were incubated in AIP monoclonal antibody (Product # MA3-16515). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of AIP in immersion fixed paraffin-embedded sections of human spleen. Samples were incubated in AIP monoclonal antibody (Product # MA3-16515) using a dilution of 1:300 for 1 hour at room temperature followed by the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in splenocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of AIP in 1 x 10^6 CHO (A) and MCF-7 (B) cells. Samples were incubated in AIP monoclonal antibody (Product # MA3-16515) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.
