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LearnNBP1-32870
antibody from Novus Biologicals
Targeting: EXOSC10
p2, p3, p4, PM-Scl, PM/Scl-100, PMSCL2, RRP6, Rrp6p
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- NBP1-32870 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32870, RRID:AB_2262550
- Product name
- Rabbit Polyclonal Exosome component 10 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression.
RNAP II CTD tyrosine 1 performs diverse functions in vertebrate cells.
A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage.
Ogami K, Richard P, Chen Y, Hoque M, Li W, Moresco JJ, Yates JR 3rd, Tian B, Manley JL
Genes & development 2017 Jun 15;31(12):1257-1271
Genes & development 2017 Jun 15;31(12):1257-1271
RNAP II CTD tyrosine 1 performs diverse functions in vertebrate cells.
Hsin JP, Li W, Hoque M, Tian B, Manley JL
eLife 2014 May 8;3:e02112
eLife 2014 May 8;3:e02112
A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage.
Richard P, Feng S, Manley JL
Genes & development 2013 Oct 15;27(20):2227-32
Genes & development 2013 Oct 15;27(20):2227-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE, antibody diluted at 1:3000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Exosome component 10 Antibody [NBP1-32870] - analysis of EXOSC10 in HeLa whole cell extracts treated with (1) si-control and (2) EXOSC10 siRNA using anti-EXOSC10 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
