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LearnPA5-28672
antibody from Invitrogen Antibodies
Targeting: EXOSC10
p2, p3, p4, PM-Scl, PM/Scl-100, PMSCL2, RRP6, Rrp6p
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28672 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EXOSC10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, EXOSC10-transfected 293T. Predicted reactivity: Chimpanzee (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.65 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects.
Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects.
Shukla S, Schmidt JC, Goldfarb KC, Cech TR, Parker R
Nature structural & molecular biology 2016 Apr;23(4):286-92
Nature structural & molecular biology 2016 Apr;23(4):286-92
Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects.
Shukla S, Schmidt JC, Goldfarb KC, Cech TR, Parker R
Nature structural & molecular biology 2016 Apr;23(4):286-92
Nature structural & molecular biology 2016 Apr;23(4):286-92
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EXOSC10 Polyclonal Antibody (Product # PA5-28672). Sample (30 µg of whole cell lysate). Lane A: 293T . 7.5% SDS PAGE. EXOSC10 Polyclonal Antibody (Product # PA5-28672) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PM-Scl was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PM-Scl Polyclonal Antibody (Product # PA5-28672) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Exosome component 10 (Product # PA5-28672) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EXOSC10 Polyclonal Antibody immunoprecipitates EXOSC 10 protein in IP experiments. IP Sample: 1,000 µg 293T whole cell lysate/extract A. 30 µg 293T whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of EXOSC 10 protein by 2.5 µg of EXOSC 10 antibody (Product # PA5-28672) 7.5% SDS-PAGE The immunoprecipitated EXOSC 10 protein was detected by EXOSC10 Polyclonal Antibody (Product # PA5-28672) diluted at 1:1,000.
