PAB23506

antibody from Abnova Corporation

Targeting: DDX23 prp28, PRPF28, SNRNP100, U5-100K

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23506

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23506, RRID:AB_11123385

Product name

DDX23 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant DDX23.

Antigen sequence

RNKKDRDRDKDGHRRDKDRKRSSLSPGRGKDFKSR
KDRDSKKDEEDEHGDKKPKAQPLSLEELLAKKKAE
EEAEAKPKFLSKAEREAEALKRRQQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with DDX23 polyclonal antibody (Cat # PAB23506) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with DDX23 polyclonal antibody (Cat # PAB23506) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and plasma membrane.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate with DDX23 polyclonal antibody (Cat # PAB23506) shows strong nuclear positivity in glandular cells at 1:1000-1:2500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)