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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30312 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX23 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, NIH-3T3, JC.
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX23 Polyclonal Antibody (Product # PA5-30312). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC . 7.5% SDS PAGE. DDX23 Polyclonal Antibody (Product # PA5-30312) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX23 Polyclonal Antibody (Product # PA5-30312). Sample (30 µg of whole cell lysate). Lane A: JurKat . 7.5% SDS PAGE. DDX23 Polyclonal Antibody (Product # PA5-30312) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDX23 Polyclonal Antibody detects DDX23 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDX23 protein stained by DDX23 Polyclonal Antibody (Product # PA5-30312) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded AZ521 xenograft, using DDX23 (Product # PA5-30312) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
