MAB6942

antibody from Abnova Corporation

Targeting: ITGAL CD11A, LFA-1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6942

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB6942, RRID:AB_10680302

Product name

ITGAL monoclonal antibody, clone 38

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against native ITGAL.

Isotype

IgG

Antibody clone number

38

Storage

Store at 4°C.

References

Submitted references

Analysis of the binding site on intercellular adhesion molecule 3 for the leukocyte integrin lymphocyte function-associated antigen 1.

Holness CL, Bates PA, Little AJ, Buckley CD, McDowall A, Bossy D, Hogg N, Simmons DL
The Journal of biological chemistry 1995 Jan 13;270(2):877-84

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Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.

Landis RC, McDowall A, Holness CL, Littler AJ, Simmons DL, Hogg N
The Journal of cell biology 1994 Jul;126(2):529-37

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Regulated expression of Mg2+ binding epitope on leukocyte integrin alpha subunits.

Dransfield I, Hogg N
The EMBO journal 1989 Dec 1;8(12):3759-65

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

5 X 10⁵ cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80 uL of ITGAL monoclonal antibody, clone 38 (Cat # MAB6942) at 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.

Validation comment

Flow Cytometry