301202

antibody from BioLegend

Targeting: ITGAL CD11A, LFA-1

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

301202

Provider

BioLegend

Proper citation

BioLegend Cat#301202, RRID:AB_314140

Product name

Purified anti-human CD11a

Antibody type

Monoclonal

Antigen

CD11a

Description

CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). The HI111 antibody blocks cell-cell interaction.

Reactivity

Human, Bovine, Canine, Horse, Rabbit, Sheep, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4° C.

References

Submitted references

Regulation of LFA-1-dependent inflammatory cell recruitment by Cbl-b and 14-3-3 proteins.

Choi EY, Orlova VV, Fagerholm SC, Nurmi SM, Zhang L, Ballantyne CM, Gahmberg CG, Chavakis T
Blood 2008 Apr 1;111(7):3607-14

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes stained with purified HI111, followed by anti-mouse IgGs FITC

Conjugate

Unconjugated