Antibody data
- Antibody Data
- Antigen structure
- References [51]
- Comments [0]
- Validations
- Flow cytometry [4]
- Other assay [1]
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Validation data
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- Product number
- MA11A10 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD11a Monoclonal Antibody (TS1/22)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- TS1/22
- Vial size
- 200 µg
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
Submitted references Increasing LFA-1 Expression Enhances Immune Synapse Architecture and T Cell Receptor Signaling in Jurkat E6.1 Cells.
The kinetics of E-selectin- and P-selectin-induced intermediate activation of integrin αLβ2 on neutrophils.
Anti-αLβ2 antibodies reveal novel endocytotic cross-modulatory functionality.
VLA-4 phosphorylation during tumor and immune cell migration relies on its coupling to VEGFR2 and CXCR4 by syndecan-1.
PRDM4 mediates YAP-induced cell invasion by activating leukocyte-specific integrin β2 expression.
LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin.
Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell-mediated cytotoxicity.
Dynamic alterations in chemokine gradients induce transendothelial shuttling of human T cells under physiologic shear conditions.
Inflamed lymphatic endothelium suppresses dendritic cell maturation and function via Mac-1/ICAM-1-dependent mechanism.
Dual mode of HMG-CoA reductase inhibition on dendritic cell invasion.
Adhesion of Epstein-Barr virus-positive natural killer cell lines to cultured endothelial cells stimulated with inflammatory cytokines.
High-mobility group box 1 activates integrin-dependent homing of endothelial progenitor cells.
Adhesion of peripheral blood mononuclear cells and CD4+ T cells from patients with psoriasis to cultured endothelial cells via the interaction between lymphocyte function-associated antigen type 1 and intercellular adhesion molecule 1.
Recruitment of dynein to the Jurkat immunological synapse.
A novel flow-cytometry-based assay for quantification of affinity and avidity changes of integrins.
Human macrophages rescue myoblasts and myotubes from apoptosis through a set of adhesion molecular systems.
Talin1 regulates TCR-mediated LFA-1 function.
Talin1 regulates TCR-mediated LFA-1 function.
Dendritic cell adhesion is enhanced on endothelial cells preexposed to calcineurin inhibitors.
Dendritic cell adhesion is enhanced on endothelial cells preexposed to calcineurin inhibitors.
Monocyte adhesion to xenogeneic endothelium during laminar flow is dependent on alpha-Gal-mediated monocyte activation.
Monocyte adhesion to xenogeneic endothelium during laminar flow is dependent on alpha-Gal-mediated monocyte activation.
Human CD1-restricted T cell recognition of lipids from pollens.
Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils.
Transendothelial migration of human basophils.
Phenyl methimazole inhibits TNF-alpha-induced VCAM-1 expression in an IFN regulatory factor-1-dependent manner and reduces monocytic cell adhesion to endothelial cells.
Human natural killer cell activity against porcine targets: modulation by control of the oxidation-reduction environment and role of adhesion molecule interactions.
Human natural killer cell activity against porcine targets: modulation by control of the oxidation-reduction environment and role of adhesion molecule interactions.
Vav1 phosphorylation is induced by beta2 integrin engagement on natural killer cells upstream of actin cytoskeleton and lipid raft reorganization.
Endothelial determinants of dendritic cell adhesion and migration: new implications for vascular diseases.
CD2 molecules redistribute to the uropod during T cell scanning: implications for cellular activation and immune surveillance.
Superantigen-induced T cell:B cell conjugation is mediated by LFA-1 and requires signaling through Lck, but not ZAP-70.
Different roles of the CD2 and LFA-1 T-cell co-receptors for regulating cytotoxic, proliferative, and cytokine responses of human V gamma 9/V delta 2 T cells.
Alpha 4 beta 1 integrin/VCAM-1 interaction activates alpha L beta 2 integrin-mediated adhesion to ICAM-1 in human T cells.
CD11b/CD18-coated microspheres attach to E-selectin under flow.
CD11b/CD18-coated microspheres attach to E-selectin under flow.
ICAM-3 (CD50) is expressed by human mast cells: induction of homotypic mast cell aggregation via ICAM-3.
ICAM-3 (CD50) is expressed by human mast cells: induction of homotypic mast cell aggregation via ICAM-3.
Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1.
Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1.
Differential requirements for LFA-1 binding to ICAM-1 and LFA-1-mediated cell aggregation.
Fever-range hyperthermia enhances L-selectin-dependent adhesion of lymphocytes to vascular endothelium.
Respiratory syncytial virus infection enhances neutrophil and eosinophil adhesion to cultured respiratory epithelial cells. Roles of CD18 and intercellular adhesion molecule-1.
Peripheral V gamma 9/V delta 2 T cell deletion and anergy to nonpeptidic mycobacterial antigens in asymptomatic HIV-1-infected persons.
Isolation and characterization of cell lines with genetically distinct mutations downstream of protein kinase C that result in defective activation-dependent regulation of T cell integrin function.
Intercellular adhesion molecule-2 (CD102) binds to the leukocyte integrin CD11b/CD18 through the A domain.
Immunohistologic analysis of the distribution of cell adhesion molecules within the inflammatory synovial microenvironment.
Heterogeneous mutations in the beta subunit common to the LFA-1, Mac-1, and p150,95 glycoproteins cause leukocyte adhesion deficiency.
Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family.
A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
Cassioli C, Balint S, Compeer EB, Felce JH, Gamberucci A, Della Bella C, Felce SL, Brunetti J, Valvo S, Pende D, D'Elios MM, Moretta L, Dustin ML, Baldari CT
Frontiers in cell and developmental biology 2021;9:673446
Frontiers in cell and developmental biology 2021;9:673446
The kinetics of E-selectin- and P-selectin-induced intermediate activation of integrin αLβ2 on neutrophils.
Zhou F, Zhang F, Zarnitsyna VI, Doudy L, Yuan Z, Li K, McEver RP, Lu H, Zhu C
Journal of cell science 2021 Sep 15;134(18)
Journal of cell science 2021 Sep 15;134(18)
Anti-αLβ2 antibodies reveal novel endocytotic cross-modulatory functionality.
Mancuso RV, Casper J, Schmidt AG, Krähenbühl S, Weitz-Schmidt G
British journal of pharmacology 2020 Jun;177(12):2696-2711
British journal of pharmacology 2020 Jun;177(12):2696-2711
VLA-4 phosphorylation during tumor and immune cell migration relies on its coupling to VEGFR2 and CXCR4 by syndecan-1.
Jung O, Beauvais DM, Adams KM, Rapraeger AC
Journal of cell science 2019 Oct 28;132(20)
Journal of cell science 2019 Oct 28;132(20)
PRDM4 mediates YAP-induced cell invasion by activating leukocyte-specific integrin β2 expression.
Liu H, Dai X, Cao X, Yan H, Ji X, Zhang H, Shen S, Si Y, Zhang H, Chen J, Li L, Zhao JC, Yu J, Feng XH, Zhao B
EMBO reports 2018 Jun;19(6)
EMBO reports 2018 Jun;19(6)
LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin.
Kawamoto E, Okamoto T, Takagi Y, Honda G, Suzuki K, Imai H, Shimaoka M
Biochemical and biophysical research communications 2016 May 13;473(4):1005-1012
Biochemical and biophysical research communications 2016 May 13;473(4):1005-1012
Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell-mediated cytotoxicity.
Tuli A, Thiery J, James AM, Michelet X, Sharma M, Garg S, Sanborn KB, Orange JS, Lieberman J, Brenner MB
Molecular biology of the cell 2013 Dec;24(23):3721-35
Molecular biology of the cell 2013 Dec;24(23):3721-35
Dynamic alterations in chemokine gradients induce transendothelial shuttling of human T cells under physiologic shear conditions.
Lee JY, Buzney CD, Poznansky MC, Sackstein R
Journal of leukocyte biology 2009 Dec;86(6):1285-94
Journal of leukocyte biology 2009 Dec;86(6):1285-94
Inflamed lymphatic endothelium suppresses dendritic cell maturation and function via Mac-1/ICAM-1-dependent mechanism.
Podgrabinska S, Kamalu O, Mayer L, Shimaoka M, Snoeck H, Randolph GJ, Skobe M
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 1;183(3):1767-79
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 1;183(3):1767-79
Dual mode of HMG-CoA reductase inhibition on dendritic cell invasion.
Kofler S, Schlichting C, Jankl S, Nickel T, Weis M
Atherosclerosis 2008 Mar;197(1):105-10
Atherosclerosis 2008 Mar;197(1):105-10
Adhesion of Epstein-Barr virus-positive natural killer cell lines to cultured endothelial cells stimulated with inflammatory cytokines.
Kanno H, Watabe D, Shimizu N, Sawai T
Clinical and experimental immunology 2008 Mar;151(3):519-27
Clinical and experimental immunology 2008 Mar;151(3):519-27
High-mobility group box 1 activates integrin-dependent homing of endothelial progenitor cells.
Chavakis E, Hain A, Vinci M, Carmona G, Bianchi ME, Vajkoczy P, Zeiher AM, Chavakis T, Dimmeler S
Circulation research 2007 Feb 2;100(2):204-12
Circulation research 2007 Feb 2;100(2):204-12
Adhesion of peripheral blood mononuclear cells and CD4+ T cells from patients with psoriasis to cultured endothelial cells via the interaction between lymphocyte function-associated antigen type 1 and intercellular adhesion molecule 1.
Watabe D, Kanno H, Yoshida A, Kurose A, Akasaka T, Sawai T
The British journal of dermatology 2007 Aug;157(2):259-65
The British journal of dermatology 2007 Aug;157(2):259-65
Recruitment of dynein to the Jurkat immunological synapse.
Combs J, Kim SJ, Tan S, Ligon LA, Holzbaur EL, Kuhn J, Poenie M
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 3;103(40):14883-8
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 3;103(40):14883-8
A novel flow-cytometry-based assay for quantification of affinity and avidity changes of integrins.
Konstandin MH, Sester U, Klemke M, Weschenfelder T, Wabnitz GH, Samstag Y
Journal of immunological methods 2006 Mar 20;310(1-2):67-77
Journal of immunological methods 2006 Mar 20;310(1-2):67-77
Human macrophages rescue myoblasts and myotubes from apoptosis through a set of adhesion molecular systems.
Sonnet C, Lafuste P, Arnold L, Brigitte M, Poron F, Authier FJ, Chrétien F, Gherardi RK, Chazaud B
Journal of cell science 2006 Jun 15;119(Pt 12):2497-507
Journal of cell science 2006 Jun 15;119(Pt 12):2497-507
Talin1 regulates TCR-mediated LFA-1 function.
Simonson WT, Franco SJ, Huttenlocher A
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):7707-14
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):7707-14
Talin1 regulates TCR-mediated LFA-1 function.
Simonson WT, Franco SJ, Huttenlocher A
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):7707-14
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):7707-14
Dendritic cell adhesion is enhanced on endothelial cells preexposed to calcineurin inhibitors.
Schlichting CL, Schareck WD, Weis M
Journal of cardiovascular pharmacology 2005 Sep;46(3):250-4
Journal of cardiovascular pharmacology 2005 Sep;46(3):250-4
Dendritic cell adhesion is enhanced on endothelial cells preexposed to calcineurin inhibitors.
Schlichting CL, Schareck WD, Weis M
Journal of cardiovascular pharmacology 2005 Sep;46(3):250-4
Journal of cardiovascular pharmacology 2005 Sep;46(3):250-4
Monocyte adhesion to xenogeneic endothelium during laminar flow is dependent on alpha-Gal-mediated monocyte activation.
Peterson MD, Jin R, Hyduk S, Duchesneau P, Cybulsky MI, Waddell TK
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 15;174(12):8072-81
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 15;174(12):8072-81
Monocyte adhesion to xenogeneic endothelium during laminar flow is dependent on alpha-Gal-mediated monocyte activation.
Peterson MD, Jin R, Hyduk S, Duchesneau P, Cybulsky MI, Waddell TK
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 15;174(12):8072-81
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 15;174(12):8072-81
Human CD1-restricted T cell recognition of lipids from pollens.
Agea E, Russano A, Bistoni O, Mannucci R, Nicoletti I, Corazzi L, Postle AD, De Libero G, Porcelli SA, Spinozzi F
The Journal of experimental medicine 2005 Jul 18;202(2):295-308
The Journal of experimental medicine 2005 Jul 18;202(2):295-308
Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils.
Zen K, Utech M, Liu Y, Soto I, Nusrat A, Parkos CA
The Journal of biological chemistry 2004 Oct 22;279(43):44924-30
The Journal of biological chemistry 2004 Oct 22;279(43):44924-30
Transendothelial migration of human basophils.
Iikura M, Ebisawa M, Yamaguchi M, Tachimoto H, Ohta K, Yamamoto K, Hirai K
Journal of immunology (Baltimore, Md. : 1950) 2004 Oct 15;173(8):5189-95
Journal of immunology (Baltimore, Md. : 1950) 2004 Oct 15;173(8):5189-95
Phenyl methimazole inhibits TNF-alpha-induced VCAM-1 expression in an IFN regulatory factor-1-dependent manner and reduces monocytic cell adhesion to endothelial cells.
Dagia NM, Harii N, Meli AE, Sun X, Lewis CJ, Kohn LD, Goetz DJ
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2041-9
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2041-9
Human natural killer cell activity against porcine targets: modulation by control of the oxidation-reduction environment and role of adhesion molecule interactions.
Horvath-Arcidiacono JA, Tsuyuki S, Mostowski H, Bloom ET
Cellular immunology 2003 Mar;222(1):35-44
Cellular immunology 2003 Mar;222(1):35-44
Human natural killer cell activity against porcine targets: modulation by control of the oxidation-reduction environment and role of adhesion molecule interactions.
Horvath-Arcidiacono JA, Tsuyuki S, Mostowski H, Bloom ET
Cellular immunology 2003 Mar;222(1):35-44
Cellular immunology 2003 Mar;222(1):35-44
Vav1 phosphorylation is induced by beta2 integrin engagement on natural killer cells upstream of actin cytoskeleton and lipid raft reorganization.
Riteau B, Barber DF, Long EO
The Journal of experimental medicine 2003 Aug 4;198(3):469-74
The Journal of experimental medicine 2003 Aug 4;198(3):469-74
Endothelial determinants of dendritic cell adhesion and migration: new implications for vascular diseases.
Weis M, Schlichting CL, Engleman EG, Cooke JP
Arteriosclerosis, thrombosis, and vascular biology 2002 Nov 1;22(11):1817-23
Arteriosclerosis, thrombosis, and vascular biology 2002 Nov 1;22(11):1817-23
CD2 molecules redistribute to the uropod during T cell scanning: implications for cellular activation and immune surveillance.
Tibaldi EV, Salgia R, Reinherz EL
Proceedings of the National Academy of Sciences of the United States of America 2002 May 28;99(11):7582-7
Proceedings of the National Academy of Sciences of the United States of America 2002 May 28;99(11):7582-7
Superantigen-induced T cell:B cell conjugation is mediated by LFA-1 and requires signaling through Lck, but not ZAP-70.
Morgan MM, Labno CM, Van Seventer GA, Denny MF, Straus DB, Burkhardt JK
Journal of immunology (Baltimore, Md. : 1950) 2001 Nov 15;167(10):5708-18
Journal of immunology (Baltimore, Md. : 1950) 2001 Nov 15;167(10):5708-18
Different roles of the CD2 and LFA-1 T-cell co-receptors for regulating cytotoxic, proliferative, and cytokine responses of human V gamma 9/V delta 2 T cells.
Wang P, Malkovsky M
Molecular medicine (Cambridge, Mass.) 2000 Mar;6(3):196-207
Molecular medicine (Cambridge, Mass.) 2000 Mar;6(3):196-207
Alpha 4 beta 1 integrin/VCAM-1 interaction activates alpha L beta 2 integrin-mediated adhesion to ICAM-1 in human T cells.
Chan JR, Hyduk SJ, Cybulsky MI
Journal of immunology (Baltimore, Md. : 1950) 2000 Jan 15;164(2):746-53
Journal of immunology (Baltimore, Md. : 1950) 2000 Jan 15;164(2):746-53
CD11b/CD18-coated microspheres attach to E-selectin under flow.
Crutchfield KL, Shinde Patil VR, Campbell CJ, Parkos CA, Allport JR, Goetz DJ
Journal of leukocyte biology 2000 Feb;67(2):196-205
Journal of leukocyte biology 2000 Feb;67(2):196-205
CD11b/CD18-coated microspheres attach to E-selectin under flow.
Crutchfield KL, Shinde Patil VR, Campbell CJ, Parkos CA, Allport JR, Goetz DJ
Journal of leukocyte biology 2000 Feb;67(2):196-205
Journal of leukocyte biology 2000 Feb;67(2):196-205
ICAM-3 (CD50) is expressed by human mast cells: induction of homotypic mast cell aggregation via ICAM-3.
Babina M, Mammeri K, Henz BM
Cell adhesion and communication 1999;7(3):195-209
Cell adhesion and communication 1999;7(3):195-209
ICAM-3 (CD50) is expressed by human mast cells: induction of homotypic mast cell aggregation via ICAM-3.
Babina M, Mammeri K, Henz BM
Cell adhesion and communication 1999;7(3):195-209
Cell adhesion and communication 1999;7(3):195-209
Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1.
Edwards CP, Fisher KL, Presta LG, Bodary SC
The Journal of biological chemistry 1998 Oct 30;273(44):28937-44
The Journal of biological chemistry 1998 Oct 30;273(44):28937-44
Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1.
Edwards CP, Fisher KL, Presta LG, Bodary SC
The Journal of biological chemistry 1998 Oct 30;273(44):28937-44
The Journal of biological chemistry 1998 Oct 30;273(44):28937-44
Differential requirements for LFA-1 binding to ICAM-1 and LFA-1-mediated cell aggregation.
Petruzzelli L, Maduzia L, Springer TA
Journal of immunology (Baltimore, Md. : 1950) 1998 May 1;160(9):4208-16
Journal of immunology (Baltimore, Md. : 1950) 1998 May 1;160(9):4208-16
Fever-range hyperthermia enhances L-selectin-dependent adhesion of lymphocytes to vascular endothelium.
Wang WC, Goldman LM, Schleider DM, Appenheimer MM, Subjeck JR, Repasky EA, Evans SS
Journal of immunology (Baltimore, Md. : 1950) 1998 Jan 15;160(2):961-9
Journal of immunology (Baltimore, Md. : 1950) 1998 Jan 15;160(2):961-9
Respiratory syncytial virus infection enhances neutrophil and eosinophil adhesion to cultured respiratory epithelial cells. Roles of CD18 and intercellular adhesion molecule-1.
Stark JM, Godding V, Sedgwick JB, Busse WW
Journal of immunology (Baltimore, Md. : 1950) 1996 Jun 15;156(12):4774-82
Journal of immunology (Baltimore, Md. : 1950) 1996 Jun 15;156(12):4774-82
Peripheral V gamma 9/V delta 2 T cell deletion and anergy to nonpeptidic mycobacterial antigens in asymptomatic HIV-1-infected persons.
Poccia F, Boullier S, Lecoeur H, Cochet M, Poquet Y, Colizzi V, Fournie JJ, Gougeon ML
Journal of immunology (Baltimore, Md. : 1950) 1996 Jul 1;157(1):449-61
Journal of immunology (Baltimore, Md. : 1950) 1996 Jul 1;157(1):449-61
Isolation and characterization of cell lines with genetically distinct mutations downstream of protein kinase C that result in defective activation-dependent regulation of T cell integrin function.
Mobley JL, Ennis E, Shimizu Y
Journal of immunology (Baltimore, Md. : 1950) 1996 Feb 1;156(3):948-56
Journal of immunology (Baltimore, Md. : 1950) 1996 Feb 1;156(3):948-56
Intercellular adhesion molecule-2 (CD102) binds to the leukocyte integrin CD11b/CD18 through the A domain.
Xie J, Li R, Kotovuori P, Vermot-Desroches C, Wijdenes J, Arnaout MA, Nortamo P, Gahmberg CG
Journal of immunology (Baltimore, Md. : 1950) 1995 Oct 1;155(7):3619-28
Journal of immunology (Baltimore, Md. : 1950) 1995 Oct 1;155(7):3619-28
Immunohistologic analysis of the distribution of cell adhesion molecules within the inflammatory synovial microenvironment.
Hale LP, Martin ME, McCollum DE, Nunley JA, Springer TA, Singer KH, Haynes BF
Arthritis and rheumatism 1989 Jan;32(1):22-30
Arthritis and rheumatism 1989 Jan;32(1):22-30
Heterogeneous mutations in the beta subunit common to the LFA-1, Mac-1, and p150,95 glycoproteins cause leukocyte adhesion deficiency.
Kishimoto TK, Hollander N, Roberts TM, Anderson DC, Springer TA
Cell 1987 Jul 17;50(2):193-202
Cell 1987 Jul 17;50(2):193-202
Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family.
Kishimoto TK, O'Connor K, Lee A, Roberts TM, Springer TA
Cell 1987 Feb 27;48(4):681-90
Cell 1987 Feb 27;48(4):681-90
A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
Sanchez-Madrid F, Nagy JA, Robbins E, Simon P, Springer TA
The Journal of experimental medicine 1983 Dec 1;158(6):1785-1803
The Journal of experimental medicine 1983 Dec 1;158(6):1785-1803
A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
Sanchez-Madrid F, Nagy JA, Robbins E, Simon P, Springer TA
The Journal of experimental medicine 1983 Dec 1;158(6):1785-1803
The Journal of experimental medicine 1983 Dec 1;158(6):1785-1803
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD11 alpha in BAF-3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD11 alpha monoclonal antibody (Product # MA11A10) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD11 alpha in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA11A10) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD11 alpha in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA11A10) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CD11a/ITGAL (red histogram) on Jurkat cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with 4 µg of a CD11a/ITGAL monoclonal antibody (Product # MA11A10) or with PBS alone (black histogram) for 1 hour at room temperature. For flow cytometry analysis, 30-minute incubation with DyLight 650 goat anti-mouse IgG secondary antibody (Product # 84545) at a dilution of 1:250 was performed and 15,000 cells were acquired for each sample.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL