14822-1-AP

antibody from Invitrogen Antibodies

Targeting: GIPC1 C19orf3, GIPC, GLUT1CBP, Hs.6454, NIP, RGS19IP1, SEMCAP, SYNECTIN, TIP-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14822-1-AP

Provider

Invitrogen Antibodies

Product name

GIPC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPLGLGRRK KAPPLVENEE AEPGRGGLGV GEPGPLGGGG SGGPQMGLPP PPPALRPRLV FHTQLAHGSP TGRIEGFTNV KELYGKIAEA FRLPTAEVMF CTLNTHKVDM DKLLGGQIGL EDFIFAHVKG QRKEVEVFKS EDALGLTITD NGAGYAFIKR IKEGSVIDHI HLISVGDMIE AINGQSLLGC RHYEVARLLK ELPRGRTFTL KLTEPRKAFD MISQRSAGGR PGSGPQLGTG RGTLRLRSRG PATVEDLPSA FEEKAIEKVD DLLESYMGIR DTELAATMVE LGKDKRNPDE LAEALDERLG DFAFPDEFVF DVWGAIGDAK VGRY (1-333 aa encoded by BC000410)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.41 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 14822-1-AP (GIPC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A2780 cells, using 14822-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14822-1-AP (GIPC1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14822-1-AP (GIPC1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GIPC1 (IP:14822-1-AP, 4ug; Detection:14822-1-AP 1:500) with mouse brain tissue lysate 9500ug.