MA5-32422

antibody from Invitrogen Antibodies

Targeting: HDAC8 HDACL1, KDAC8, MRXS6, RPD3, WTS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32422

Provider

Invitrogen Antibodies

Product name

HDAC8 Recombinant Rabbit Monoclonal Antibody (JJ0845)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JJ0845

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HDAC8 in different lysates using a Monoclonal antibody (Product #MA5-32422) at a dilution of 1:1,000. Positive control: Lane 1: Hela, Lane 2: K562.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of HDAC8 in A549 cells using a HDAC8 Monoclonal antibody (Product # MA5-32422) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of HDAC8 in HepG2 cells using a HDAC8 Monoclonal antibody (Product # MA5-32422) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of HDAC8 in Hela cells using a HDAC8 Monoclonal antibody (Product # MA5-32422) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of HDAC8 of paraffin-embedded Human lung cancer tissue using a HDAC8 Monoclonal antibody (Product #MA5-32422). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of HDAC8 of paraffin-embedded Human kidney tissue using a HDAC8 Monoclonal antibody (Product #MA5-32422). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of HDAC8 of paraffin-embedded Human pancreas tissue using a HDAC8 Monoclonal antibody (Product #MA5-32422). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of HDAC8 of paraffin-embedded Human lung tissue using a HDAC8 Monoclonal antibody (Product #MA5-32422). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of HDAC8 in K562 cells using a HDAC8 Monoclonal Antibody (Product # MA5-32422) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.