21702-1-AP

antibody from Invitrogen Antibodies

Targeting: HLA-DRB5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

21702-1-AP

Provider

Invitrogen Antibodies

Product name

HLA-DRB5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MVCLKLPGG SYMAKLTVTL MVLSSPLALA GDTRPRFLQQ DKYECHFFNG TERVRFLHRD IYNQEEDLRF DSDVGEYRAV TELGRPDAEY WNSQKDFLED RRAAVDTYCR HNYGVGESFT VQRRVEPKVT VYPARTQTLQ HHNLLVCSVN GFYPGSIEVR WFRNSQEEKA GVVSTGLIQN GDWTFQTLVM LETVPRSGEV YTCQVEHPSV TSPLTVEWRA QSESAQSKML SGVGGFVLGL LFLGAGLFIY FKNQKGHSGL HPTGLVS (1-266 aa encoded by BC009234)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HL-60 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 Raji cells were stained with 0.20 µg HLA-DRB5 antibody (Product # 21702-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.