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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10070 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10070, RRID:AB_1676245
- Product name
- PARK7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PARK7.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Reduced anti-oxidative stress activities of DJ-1 mutants found in Parkinson's disease patients.
Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism.
DJBP: a novel DJ-1-binding protein, negatively regulates the androgen receptor by recruiting histone deacetylase complex, and DJ-1 antagonizes this inhibition by abrogation of this complex.
Takahashi-Niki K, Niki T, Taira T, Iguchi-Ariga SM, Ariga H
Biochemical and biophysical research communications 2004 Jul 23;320(2):389-97
Biochemical and biophysical research communications 2004 Jul 23;320(2):389-97
Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism.
Bonifati V, Rizzu P, van Baren MJ, Schaap O, Breedveld GJ, Krieger E, Dekker MC, Squitieri F, Ibanez P, Joosse M, van Dongen JW, Vanacore N, van Swieten JC, Brice A, Meco G, van Duijn CM, Oostra BA, Heutink P
Science (New York, N.Y.) 2003 Jan 10;299(5604):256-9
Science (New York, N.Y.) 2003 Jan 10;299(5604):256-9
DJBP: a novel DJ-1-binding protein, negatively regulates the androgen receptor by recruiting histone deacetylase complex, and DJ-1 antagonizes this inhibition by abrogation of this complex.
Niki T, Takahashi-Niki K, Taira T, Iguchi-Ariga SM, Ariga H
Molecular cancer research : MCR 2003 Feb;1(4):247-61
Molecular cancer research : MCR 2003 Feb;1(4):247-61
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis is shown using PARK7 polyclonal antibody (Cat # PAB10070) to detect PARK7 present in Jurkat whole cell lysate.This western blot shows reactivity with human PARK7 protein.Comparison to a molecular weight marker indicates a predominant band of ~28.0 KDa.Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown).A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose.The blot was incubated with a 1:1,300 dilution ofthe antibody overnight at 4°C followed by detectionusing IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at RT.IRDye™800fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDyeis a trademark of LI-COR, Inc.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of PARK7 polyclonal antibody (Cat # PAB10070) was used at a 5 ug/mL to detect PARK7 in a variety of tissues.In some tissues elevated background stainingwas noted.In these instances further optimization of dilution is suggested.This image shows PARK7 staining of human pancreas.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Tina Roush,Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
