- NB300-270 - Novus Biologicals PARK7 antibody

Submitted references Dysregulation in the Brain Protein Profile of Zebrafish Lacking the Parkinson's Disease-Related Protein DJ-1.

Edson AJ, Hushagen HA, Frøyset AK, Elda I, Khan EA, Di Stefano A, Fladmark KE
Molecular neurobiology 2019 Dec;56(12):8306-8322

Mild Inflammatory Profile without Gliosis in the c-Rel Deficient Mouse Modeling a Late-Onset Parkinsonism.

Porrini V, Mota M, Parrella E, Bellucci A, Benarese M, Faggi L, Tonin P, Spano PF, Pizzi M
Frontiers in aging neuroscience 2017;9:229

Loss of DJ-1 elicits retinal abnormalities, visual dysfunction, and increased oxidative stress in mice.

Bonilha VL, Bell BA, Rayborn ME, Yang X, Kaul C, Grossman GH, Samuels IS, Hollyfield JG, Xie C, Cai H, Shadrach KG
Experimental eye research 2015 Oct;139:22-36

Identification of the Protective Role of DJ-1 in Hypoglycemic Astrocyte Injury Using Proteomics.

Deng J, Zhao F, Yu X, Li D, Zhao Y
Journal of proteome research 2015 Jul 2;14(7):2839-48

Transnitrosylation from DJ-1 to PTEN attenuates neuronal cell death in parkinson's disease models.

Choi MS, Nakamura T, Cho SJ, Han X, Holland EA, Qu J, Petsko GA, Yates JR 3rd, Liddington RC, Lipton SA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Nov 5;34(45):15123-31

DJ-1-dependent regulation of oxidative stress in the retinal pigment epithelium (RPE).

Shadrach KG, Rayborn ME, Hollyfield JG, Bonilha VL
PloS one 2013;8(7):e67983

DJ-1 cooperates with PYCR1 in cell protection against oxidative stress.

Yasuda T, Kaji Y, Agatsuma T, Niki T, Arisawa M, Shuto S, Ariga H, Iguchi-Ariga SM
Biochemical and biophysical research communications 2013 Jun 28;436(2):289-94

Chicken DT40 cell line lacking DJ-1, the gene responsible for familial Parkinson's disease, displays mitochondrial dysfunction.

Minakawa EN, Yamakado H, Tanaka A, Uemura K, Takeda S, Takahashi R
Neuroscience research 2013 Dec;77(4):228-33

DJ-1 null dopaminergic neuronal cells exhibit defects in mitochondrial function and structure: involvement of mitochondrial complex I assembly.

Heo JY, Park JH, Kim SJ, Seo KS, Han JS, Lee SH, Kim JM, Park JI, Park SK, Lim K, Hwang BD, Shong M, Kweon GR
PloS one 2012;7(3):e32629

DJ-1 isoforms in whole blood as potential biomarkers of Parkinson disease.

Lin X, Cook TJ, Zabetian CP, Leverenz JB, Peskind ER, Hu SC, Cain KC, Pan C, Edgar JS, Goodlett DR, Racette BA, Checkoway H, Montine TJ, Shi M, Zhang J
Scientific reports 2012;2:954

Role of renal DJ-1 in the pathogenesis of hypertension associated with increased reactive oxygen species production.

Cuevas S, Zhang Y, Yang Y, Escano C, Asico L, Jones JE, Armando I, Jose PA
Hypertension (Dallas, Tex. : 1979) 2012 Feb;59(2):446-52

DJ-1 associates with synaptic membranes.

Usami Y, Hatano T, Imai S, Kubo S, Sato S, Saiki S, Fujioka Y, Ohba Y, Sato F, Funayama M, Eguchi H, Shiba K, Ariga H, Shen J, Hattori N
Neurobiology of disease 2011 Sep;43(3):651-62

CD147 (Basigin/Emmprin) identifies FoxP3+CD45RO+CTLA4+-activated human regulatory T cells.

Solstad T, Bains SJ, Landskron J, Aandahl EM, Thiede B, Taskén K, Torgersen KM
Blood 2011 Nov 10;118(19):5141-51

DJ-1 mediates paraquat-induced dopaminergic neuronal cell death.

Kwon HJ, Heo JY, Shim JH, Park JH, Seo KS, Ryu MJ, Han JS, Shong M, Son JH, Kweon GR
Toxicology letters 2011 Apr 25;202(2):85-92

Increased interaction between DJ-1 and the Mi-2/ nucleosome remodelling and deacetylase complex during cellular stress.

Opsahl JA, Hjørnevik LV, Bull VH, Fismen L, Frøyset AK, Gromyko D, Solstad T, Fladmark KE
Proteomics 2010 Apr;10(7):1494-504

Identification of novel proteins associated with both alpha-synuclein and DJ-1.

Jin J, Li GJ, Davis J, Zhu D, Wang Y, Pan C, Zhang J
Molecular & cellular proteomics : MCP 2007 May;6(5):845-59

No comments: Submit comment

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: Park7/DJ-1 Antibody [NB300-270] - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Park7/DJ-1 Antibody [NB300-270] - Cells were transfected with the pCMV6-ENTRY control (lane 1) or pCMV6-ENTRY PARK7 cDNA (lane 2) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Park7(DJ-1).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Park7/DJ-1 Antibody [NB300-270] - Human HEK293 cells and mouse brain lysates.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Park7/DJ-1 Antibody [NB300-270] - ARPE-19 and D407 monolayers were treated with increasing concentrations (0 to 800 uM) of H2O2 for 18 hrs (B), harvested, and analyzed by immunoblot assay with DJ-1 antibody (upper panel). Each lane contained 20 ug of protein. Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). A representative Western is shown. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY licence

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Park7/DJ-1 Antibody [NB300-270] - Lysates of the human RPE cell lines ARPE-19 (lane 1) and D407 (lane 2), the mouse RPE cell line B6-RPE07 (lane 3), mouse primary RPE (lane 4) and mouse brain lysates (lane 5) were harvested and analyzed by immunoblot assay with DJ-1 antibody. The DJ-1 signal varied in intensity in each RPE cell culture. Each lane contained 20 ug of protein. Protein loads were confirmed in replicate blots probed with GAPDH. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB300-270] - Park7(DJ-1) detected in paraffin embedded human cortex.

NB300-270

Antibody data