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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF1620 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Wnt-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Wnt-1 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt-3a and rmWnt-5a is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q3UR96- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Calmodulin-dependent protein kinase II/cAMP response element-binding protein/Wnt/β-catenin signaling cascade regulates angiotensin II-induced podocyte injury and albuminuria.
Jiang L, Xu L, Song Y, Li J, Mao J, Zhao AZ, He W, Yang J, Dai C
The Journal of biological chemistry 2013 Aug 9;288(32):23368-79
The Journal of biological chemistry 2013 Aug 9;288(32):23368-79
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Wnt-1 in Mouse Embryonic Spinal Cord. Wnt-1 was detected in immersion fixed frozen sections of mouse embryonic spinal cord (13 d.p.c.) using 15 µg/mL Goat Anti-Mouse Wnt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1620) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
