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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 600-401-A37 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-A37, RRID:AB_2217024
- Product name
- Anti-Wnt1 (RABBIT) Antibody - 600-401-A37
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Wnt pathway activation in long term remnant rat model.
Banon-Maneus E, Rovira J, Ramirez-Bajo MJ, Moya-Rull D, Hierro-Garcia N, Takenaka S, Diekmann F, Eickelberg O, Königshoff M, Campistol JM
BioMed research international 2014;2014:324713
BioMed research international 2014;2014:324713
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-Wnt1 antibody shows detection of endogenous Wnt1 in human-derived MCF7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kDa, indicated by the arrowhead, corresponds to Wnt1. After transfer, the membrane was blocked with 5% BLOTTO. Primary antibody was used at a 1:1,400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt1 (data not shown).
- Validation comment
- Western Blot
