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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- 15116-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSD17B4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GSGVVIIMD VYSYSEKELI CHNQFSLFLV GSGGFGGKRT SDKVKVAVAI PNRPPDAVLT DTTSLNQAAL YRLSGDWNPL HIDPNFASLA GFDKPILHGL CTFGFSARRV LQQFADNDVS RFKAIKARFA KPVYPGQTLQ TEMWKEGNRI HFQTKVQETG DIVISNAYVD LAPTSGTSAK TPSEGGKLQS TFVFEEIGRR LKDIGPEVVK KVNAVFEWHI TKGGNIGAKW TIDLKSGSGK VYQGPAKGAA DTTIILSDED FMEVVLGKLD PQKAFFSGRL KARGNIMLSQ KLQMILKDYA KL (436-736 aa encoded by BC003098)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using HSD17B4 antibody 15116-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse heart using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse heart using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 15116-1-AP (HSD17B4 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 15116-1-AP (HSD17B4 antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HSD17B4 (IP:15116-1-AP, 4ug; Detection:15116-1-AP 1:500) with mouse brain tissue lysate 4000ug.
