PA5-21522

antibody from Invitrogen Antibodies

Targeting: HSD17B4 DBP, MFE-2, SDR8C1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21522

Provider

Invitrogen Antibodies

Product name

HSD17B4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Rat heart.

Concentration

1 mg/mL

References

Submitted references

Regulatory Functions of L-Carnitine, Acetyl, and Propionyl L-Carnitine in a PCOS Mouse Model: Focus on Antioxidant/Antiglycative Molecular Pathways in the Ovarian Microenvironment.

Di Emidio G, Rea F, Placidi M, Rossi G, Cocciolone D, Virmani A, Macchiarelli G, Palmerini MG, D'Alessandro AM, Artini PG, Tatone C
Antioxidants (Basel, Switzerland) 2020 Sep 15;9(9)

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Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice.

Emidio GD, Placidi M, Rea F, Rossi G, Falone S, Cristiano L, Nottola S, D'Alessandro AM, Amicarelli F, Palmerini MG, Tatone C
Cells 2020 Jan 14;9(1)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HSD17B4 Polyclonal Antibody detects HSD17B4 protein by western blot analysis. A. 20 µg Rat heart lysate/extract. 7.5% SDS-PAGE. HSD17B4 Polyclonal Antibody (Product # PA5-21522) dilution: 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 17beta-HSD4 in methanol-fixed Hep3B cells using a 17beta-HSD4 polyclonal antibody (Product # PA5-21522) at a 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed Hep3B, using HSD17B4 antibody (Product # PA5-21522) at 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of HSD17B4 was performed in paraffin-embedded rat testis tissue using HSD17B4 Polyclonal Antibody (Product # PA5-21522) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of HSD17B4 was performed in paraffin-embedded rat heart tissue using HSD17B4 Polyclonal Antibody (Product # PA5-21522) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using HSD17B4 (Product # PA5-21522) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.