MAB1995

antibody from Abnova Corporation

Targeting: MTBP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1995

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB1995, RRID:AB_1679049

Product name

MTBP monoclonal antibody, clone PHL-1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant MTBP.

Isotype

IgG

Antibody clone number

PHL-1

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Regulation of p53 and MDM2 activity by MTBP.

Brady M, Vlatkovic N, Boyd MT
Molecular and cellular biology 2005 Jan;25(2):545-53

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Assignment of the MDM2 binding protein gene (MTBP) to human chromosome band 8q24 by in situ hybridization.

Boyd MT, Zimonjic DB, Popescu NC, Athwal R, Haines DS
Cytogenetics and cell genetics 2000;90(1-2):64-5

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A novel cellular protein (MTBP) binds to MDM2 and induces a G1 arrest that is suppressed by MDM2.

Boyd MT, Vlatkovic N, Haines DS
The Journal of biological chemistry 2000 Oct 13;275(41):31883-90

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using MTBP monoclonal antibody, clone PHL-1 (Cat # MAB1995) shows detection of aband ~110 KDa corresponding to human MTBP (arrowhead).Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct.The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP.Proteins were separated bySDS-PAGE and transferred onto PDVF membrane.After blocking, the membrane was probed with the primary antibody diluted to 1 : 500 for 2h at roomtemperature followed by detection usinga Lumi-Light Plus Western Blotting Kit.