PA5-55143

antibody from Invitrogen Antibodies

Targeting: SNTB1 59-DAP, A1B, BSYN2, SNT2, SNT2B1, TIP-43

Antibody data

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Validation data

Product number: PA5-55143 - Provider product page
Provider: Invitrogen Antibodies
Product name: SNTB1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: QGCHNSAELI AEISTACTYK NQECRLTIHY ENGFSITTEP QEGAFPKTII QSPYEKLKMS SDDG
Concentration: 0.1 mg/mL

SNTB1 protein structure - PA5-55143 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Levels of SNTB1 mRNA and protein in colorectal cancer tissues. a SNTB1 mRNA expression in CRC tissues (T: n=79) and non-cancerous colorectal tissues (N: n=15) on the CRC cDNA chip was analyzed by qPCR. GAPDH was used as an internal control. * P < 0.05. b , c SNTB1 mRNA expression in CRC tissues (T) and non-cancerous colorectal tissues (N) from GEO ( b ) and TCGA ( c ) were analyzed. d , e SNTB1 protein expression in CRC tissues (T) and non-cancerous colorectal tissues (N) was determined by immunohistochemistry of a tissue microarray. Representative images were taken at magnification of 40x or 200x (right panel); the IHC score was calculated as intensity score x percentage score (left panel, see Methods). * P < 0.05. f , g Kaplan-Meier plots of survival of CRC patients, stratified by SNTB1 mRNA expression based on cDNA array ( f ) and TCGA database ( g ). Survival was analyzed with log-rank test

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 SNTB1 knockdown inhibits colorectal cancer (CRC) cell growth. HCT116 and RKO cells were transduced with lentivirus encoding either anti-SNTB1 small hairpin RNA (sh-SNTB1) or control shRNA (sh-Ctrl). a The mRNA levels of SNTB1 were determined by qPCR, GAPDH was used as an internal control. * P < 0.05. b , c The protein levels of SNTB1 were determined by Western-blot analysis. The representative images of SNTB1 and GAPDH are shown ( b ) and were quantitated using ImageLab software ( c ). GAPDH was used as an internal control and normalized to GAPDH. * P < 0.05. d The cell viability of CRC cells was determined by the CCK-8 assay. Data were normalized to the viability on Day 1 and are represented as fold change. * P < 0.05. e The colony formation assay was performed to determine cell survival. The images were obtained and the number of colonies formed were determined and normalized to the survival of control cells. * P < 0.05