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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-22217 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZHX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 0.98 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZHX2 using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ZHX2 polyclonal antibody (Product # PA5-22217) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ZHX2 Polyclonal Antibody (Product # PA5-22217). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 Polyclonal Antibody (Product # PA5-22217) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZHX2 in paraformaldehyde-fixed HeLa cells using a ZHX2 polyclonal antibody (Product # PA5-22217) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ZHX2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ZHX2 Polyclonal Antibody (Product # PA5-22217) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using ZHX2 (Product # PA5-22217) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
