PA5-22217

antibody from Invitrogen Antibodies

Targeting: ZHX2 KIAA0854

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA5-22217

Provider

Invitrogen Antibodies

Product name

ZHX2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.70 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ZHX2 using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ZHX2 polyclonal antibody (Product # PA5-22217) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ZHX2 Polyclonal Antibody (Product # PA5-22217). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 Polyclonal Antibody (Product # PA5-22217) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ZHX2 in paraformaldehyde-fixed HeLa cells using a ZHX2 polyclonal antibody (Product # PA5-22217) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ZHX2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ZHX2 Polyclonal Antibody (Product # PA5-22217) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using ZHX2 (Product # PA5-22217) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.