Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00051132-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00051132-M01, RRID:AB_2181966
- Product name
- Mouse Monoclonal RNF12 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RNF12 - ring finger protein 12
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references E3 Ubiquitin Ligase RLIM Negatively Regulates c-Myc Transcriptional Activity and Restrains Cell Proliferation.
Maintenance of Xist Imprinting Depends on Chromatin Condensation State and Rnf12 Dosage in Mice.
Round Spermatid Injection Rescues Female Lethality of a Paternally Inherited Xist Deletion in Mouse.
The role of maternal-specific H3K9me3 modification in establishing imprinted X-chromosome inactivation and embryogenesis in mice.
Nuclear receptor NR4A1 promotes breast cancer invasion and metastasis by activating TGF-β signalling.
Gao R, Wang L, Cai H, Zhu J, Yu L
PloS one 2016;11(9):e0164086
PloS one 2016;11(9):e0164086
Maintenance of Xist Imprinting Depends on Chromatin Condensation State and Rnf12 Dosage in Mice.
Fukuda A, Mitani A, Miyashita T, Sado T, Umezawa A, Akutsu H
PLoS genetics 2016 Oct;12(10):e1006375
PLoS genetics 2016 Oct;12(10):e1006375
Round Spermatid Injection Rescues Female Lethality of a Paternally Inherited Xist Deletion in Mouse.
Federici F, Magaraki A, Wassenaar E, van Veen-Buurman CJ, van de Werken C, Baart EB, Laven JS, Grootegoed JA, Gribnau J, Baarends WM
PLoS genetics 2016 Oct;12(10):e1006358
PLoS genetics 2016 Oct;12(10):e1006358
The role of maternal-specific H3K9me3 modification in establishing imprinted X-chromosome inactivation and embryogenesis in mice.
Fukuda A, Tomikawa J, Miura T, Hata K, Nakabayashi K, Eggan K, Akutsu H, Umezawa A
Nature communications 2014 Nov 14;5:5464
Nature communications 2014 Nov 14;5:5464
Nuclear receptor NR4A1 promotes breast cancer invasion and metastasis by activating TGF-β signalling.
Zhou F, Drabsch Y, Dekker TJ, de Vinuesa AG, Li Y, Hawinkels LJ, Sheppard KA, Goumans MJ, Luwor RB, de Vries CJ, Mesker WE, Tollenaar RA, Devilee P, Lu CX, Zhu H, Zhang L, Dijke PT
Nature communications 2014 Mar 3;5:3388
Nature communications 2014 Mar 3;5:3388
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF12 Antibody (1G10) [H00051132-M01] - Analysis of RNF12 expression in transfected 293T cell line by RNF12 monoclonal antibody (M01), clone 1G10.Lane 1: RNF12 transfected lysate (Predicted MW: 68.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF12 Antibody (1G10) [H00051132-M01] - RNF12 monoclonal antibody (M01), clone 1G10. Analysis of RNF12 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RNF12 Antibody (1G10) [H00051132-M01] - Detection limit for recombinant GST tagged RNF12 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RNF12 Antibody (1G10) [H00051132-M01] - Analysis of monoclonal antibody to RNF12 on HeLa cell. Antibody concentration 30 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RNF12 Antibody (1G10) [H00051132-M01] - Analysis of RNF12 transfected lysate using anti-RNF12 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RNF12 MaxPab rabbit polyclonal antibody.