LS-C799150

antibody from LSBio

Targeting: RLIM MGC15161, NY-REN-43, RNF12

Western blot (Enhanced validation)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C799150

Provider

LSBio

Product name

RLIM / RNF12 Antibody (clone OTI2D7) LS-C799150

Antibody type

Monoclonal

Description

Affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI2D7

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY RLIM. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RLIM. (1:2000)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts. (35ug) from 3 different cell lines by using anti-RLIM monoclonal antibody. (1:500)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts. (35ug) from 3 different cell lines by using anti-RLIM monoclonal antibody. (1:500)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY RLIM. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RLIM. (1:2000)