H00005261-M05

antibody from Abnova Corporation

Targeting: PHKG2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005261-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005261-M05, RRID:AB_606770

Product name

PHKG2 monoclonal antibody (M05), clone 2B8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PHKG2.

Antigen sequence

MTLDVGPEDELPDWAAAKEFYQKYDPKDVIGRGVS
SVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEV
REATRRETHILRQVAGHPHIITLIDSYESSSFMFL
VFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEA
VSFLHANNIVHRDLKPENILLDDNMQIRLSDFGFS
CHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGY
GKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMI
MEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARL
TAEQALQHPFFERCEGSQPWNLTPRQRFRVAVWTV
LAAGRVALSTHRVRPLTKNALLRDPYALRSVRHLI
DNCAFRLYGHWVKKGEQQNRAALFQHRPPGPFPIM
GPEEEGDSAAITEDEAVLVLG


Isotype

IgG

Antibody clone number

2B8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PHKG2 expression in transfected 293T cell line by PHKG2 monoclonal antibody (M05), clone 2B8.Lane 1: PHKG2 transfected lysate (Predicted MW: 46.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PHKG2 is approximately 10ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PHKG2 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol