Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-56440 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56440, RRID:AB_838547
- Product name
- Rabbit Polyclonal SMAD6 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. A 52 kDa band is observed.
- Reactivity
- Human, Mouse, Rat, Sheep, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Inhibitor of differentiation (Id) genes are expressed in the steroidogenic cells of the ovine ovary and are differentially regulated by members of the transforming growth factor-beta family.
BMP signaling in the human fetal ovary is developmentally regulated and promotes primordial germ cell apoptosis.
Ultradian oscillations of Stat, Smad, and Hes1 expression in response to serum.
Bone morphogenetic protein 2 activates Smad6 gene transcription through bone-specific transcription factor Runx2.
Low-level bisphenol A increases production of glial fibrillary acidic protein in differentiating astrocyte progenitor cells through excessive STAT3 and Smad1 activation.
Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation.
Hogg K, Etherington SL, Young JM, McNeilly AS, Duncan WC
Endocrinology 2010 Mar;151(3):1247-56
Endocrinology 2010 Mar;151(3):1247-56
BMP signaling in the human fetal ovary is developmentally regulated and promotes primordial germ cell apoptosis.
Childs AJ, Kinnell HL, Collins CS, Hogg K, Bayne RA, Green SJ, McNeilly AS, Anderson RA
Stem cells (Dayton, Ohio) 2010 Aug;28(8):1368-78
Stem cells (Dayton, Ohio) 2010 Aug;28(8):1368-78
Ultradian oscillations of Stat, Smad, and Hes1 expression in response to serum.
Yoshiura S, Ohtsuka T, Takenaka Y, Nagahara H, Yoshikawa K, Kageyama R
Proceedings of the National Academy of Sciences of the United States of America 2007 Jul 3;104(27):11292-7
Proceedings of the National Academy of Sciences of the United States of America 2007 Jul 3;104(27):11292-7
Bone morphogenetic protein 2 activates Smad6 gene transcription through bone-specific transcription factor Runx2.
Wang Q, Wei X, Zhu T, Zhang M, Shen R, Xing L, O'Keefe RJ, Chen D
The Journal of biological chemistry 2007 Apr 6;282(14):10742-8
The Journal of biological chemistry 2007 Apr 6;282(14):10742-8
Low-level bisphenol A increases production of glial fibrillary acidic protein in differentiating astrocyte progenitor cells through excessive STAT3 and Smad1 activation.
Yamaguchi H, Zhu J, Yu T, Sasaki K, Umetsu H, Kidachi Y, Ryoyama K
Toxicology 2006 Sep 21;226(2-3):131-42
Toxicology 2006 Sep 21;226(2-3):131-42
Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation.
Shen R, Chen M, Wang YJ, Kaneki H, Xing L, O'keefe RJ, Chen D
The Journal of biological chemistry 2006 Feb 10;281(6):3569-76
The Journal of biological chemistry 2006 Feb 10;281(6):3569-76
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SMAD6 Antibody [NB100-56440] - Simple Western lane view shows a specific band for SMAD6 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMAD6 Antibody [NB100-56440] - Analysis using SMAD6 antibody. Lysate from Jurkat cells probed with SMAD6 antibody at 2 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMAD6 Antibody [NB100-56440] - Analysis in mouse adult lung tissue. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMAD6 Antibody [NB100-56440] - SMAD6 was detected in immersion fixed paraffin-embedded sections of human lung using Rabbit Anti-Human SMAD6 polyclonal Antibody (Catalog # NB100-56440) at 5 ug/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Nuclear staining was localized to the pneumocytes.