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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- AF4894 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal EMR2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human EMR2 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows less than 1% cross-reactivity with recombinant mouse EMR1.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references G Protein-Coupling of Adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and Activation of G Protein Signalling by an Anti-EMR2 Antibody.
Bhudia N, Desai S, King N, Ancellin N, Grillot D, Barnes AA, Dowell SJ
Scientific reports 2020 Jan 22;10(1):1004
Scientific reports 2020 Jan 22;10(1):1004
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human EMR2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMR2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by EMR2 and Neutralization by Human EMR2 Antibody. Cell Adhesion Mediated by EMR2 and Neutralization by Human EMR2 Antibody.
