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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-30332 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF40 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, HeLa, HeLa NE.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RNF40 Polyclonal Antibody (Product # PA5-30332). Sample (30 µg of whole cell lysate). Lane A: IMR32 . 7.5% SDS PAGE. RNF40 Polyclonal Antibody (Product # PA5-30332) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RNF40 antibody detects RNF40 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNF40 antibody RNF40 Polyclonal Antibody (Product # PA5-30332) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RNF40 in paraformaldehyde-fixed MCF-7 cells using a RNF40 polyclonal antibody (Product # PA5-30332) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RNF40 (Product # PA5-30332) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RNF40 was performed in HeLa whole cell extracts using 5 µg of RNF40 Polyclonal Antibody (Product # PA5-30332). Samples were transferred to a membrane and probed with RNF40 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
