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- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Blocking/Neutralizing [1]
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- Product number
- AF-307-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal PDGFR alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse PDGF R alpha , recombinant human (rh) PDGF R beta , rhFGF R2, and rhFGF R3 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Patch repair of deep wounds by mobilized fascia.
Adaptive changes of telocytes in the urinary bladder of patients affected by neurogenic detrusor overactivity.
Morphological evidence of telocytes in human synovium.
Transcriptome analysis of PDGFRα+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFRα+ cell hyperplasia.
ACL injury reduces satellite cell abundance and promotes fibrogenic cell expansion within skeletal muscle.
Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells.
Telocytes in normal and keratoconic human cornea: an immunohistochemical and transmission electron microscopy study.
Identification and characterization of PDGFRα+ mesenchymal progenitors in human skeletal muscle.
Telocytes in Crohn's disease.
Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells.
Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection.
Distinctions between fetal and adult human platelet-derived growth factor-responsive neural precursors.
Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas.
Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
Content and activity of cAMP response element-binding protein regulate platelet-derived growth factor receptor-alpha content in vascular smooth muscles.
Correa-Gallegos D, Jiang D, Christ S, Ramesh P, Ye H, Wannemacher J, Kalgudde Gopal S, Yu Q, Aichler M, Walch A, Mirastschijski U, Volz T, Rinkevich Y
Nature 2019 Dec;576(7786):287-292
Nature 2019 Dec;576(7786):287-292
Adaptive changes of telocytes in the urinary bladder of patients affected by neurogenic detrusor overactivity.
Traini C, Fausssone-Pellegrini MS, Guasti D, Del Popolo G, Frizzi J, Serni S, Vannucchi MG
Journal of cellular and molecular medicine 2018 Jan;22(1):195-206
Journal of cellular and molecular medicine 2018 Jan;22(1):195-206
Morphological evidence of telocytes in human synovium.
Rosa I, Marini M, Guasti D, Ibba-Manneschi L, Manetti M
Scientific reports 2018 Feb 26;8(1):3581
Scientific reports 2018 Feb 26;8(1):3581
Transcriptome analysis of PDGFRα+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFRα+ cell hyperplasia.
Ha SE, Lee MY, Kurahashi M, Wei L, Jorgensen BG, Park C, Park PJ, Redelman D, Sasse KC, Becker LS, Sanders KM, Ro S
PloS one 2017;12(8):e0182265
PloS one 2017;12(8):e0182265
ACL injury reduces satellite cell abundance and promotes fibrogenic cell expansion within skeletal muscle.
Fry CS, Johnson DL, Ireland ML, Noehren B
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2017 Sep;35(9):1876-1885
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2017 Sep;35(9):1876-1885
Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells.
Daquinag AC, Dadbin A, Snyder B, Wang X, Sahin AA, Ueno NT, Kolonin MG
Molecular therapy oncolytics 2017 Sep 15;6:1-9
Molecular therapy oncolytics 2017 Sep 15;6:1-9
Telocytes in normal and keratoconic human cornea: an immunohistochemical and transmission electron microscopy study.
Marini M, Mencucci R, Rosa I, Favuzza E, Guasti D, Ibba-Manneschi L, Manetti M
Journal of cellular and molecular medicine 2017 Dec;21(12):3602-3611
Journal of cellular and molecular medicine 2017 Dec;21(12):3602-3611
Identification and characterization of PDGFRα+ mesenchymal progenitors in human skeletal muscle.
Uezumi A, Fukada S, Yamamoto N, Ikemoto-Uezumi M, Nakatani M, Morita M, Yamaguchi A, Yamada H, Nishino I, Hamada Y, Tsuchida K
Cell death & disease 2014 Apr 17;5(4):e1186
Cell death & disease 2014 Apr 17;5(4):e1186
Telocytes in Crohn's disease.
Milia AF, Ruffo M, Manetti M, Rosa I, Conte D, Fazi M, Messerini L, Ibba-Manneschi L
Journal of cellular and molecular medicine 2013 Dec;17(12):1525-36
Journal of cellular and molecular medicine 2013 Dec;17(12):1525-36
Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells.
Kusuhara S, Fukushima Y, Fukuhara S, Jakt LM, Okada M, Shimizu Y, Hata M, Nishida K, Negi A, Hirashima M, Mochizuki N, Nishikawa S, Uemura A
PloS one 2012;7(9):e45858
PloS one 2012;7(9):e45858
Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection.
Soroceanu L, Akhavan A, Cobbs CS
Nature 2008 Sep 18;455(7211):391-5
Nature 2008 Sep 18;455(7211):391-5
Distinctions between fetal and adult human platelet-derived growth factor-responsive neural precursors.
Chojnacki A, Kelly JJ, Hader W, Weiss S
Annals of neurology 2008 Aug;64(2):127-42
Annals of neurology 2008 Aug;64(2):127-42
Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
Dolloff NG, Russell MR, Loizos N, Fatatis A
Cancer research 2007 Jan 15;67(2):555-62
Cancer research 2007 Jan 15;67(2):555-62
Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
Blood 2006 Mar 1;107(5):1896-902
Blood 2006 Mar 1;107(5):1896-902
Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas.
Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K
Cancer research 2005 Aug 15;65(16):7241-8
Cancer research 2005 Aug 15;65(16):7241-8
Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH
The American journal of pathology 2003 Sep;163(3):869-78
The American journal of pathology 2003 Sep;163(3):869-78
Content and activity of cAMP response element-binding protein regulate platelet-derived growth factor receptor-alpha content in vascular smooth muscles.
Watson PA, Vinson C, Nesterova A, Reusch JE
Endocrinology 2002 Aug;143(8):2922-9
Endocrinology 2002 Aug;143(8):2922-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- PDGF R alpha in Human Breast Cancer Tissue. PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- PDGF R alpha in Human Ovary. PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- PDGF R alpha in Human Osteosarcoma. PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by PDGF-AA and Neutralization by Human PDGF R alpha Antibody. Recombinant Human PDGF-AA (Catalog # 221-AA) stimulates proliferation in the WS-1 human fetal skin fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA). The ND50 is typically 1-6 µg/mL.
