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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB3940 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3940, RRID:AB_1578542
- Product name
- PDGFRA polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PDGFRA.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Novel missense mutations in the tyrosine kinase domain of the platelet-derived growth factor receptor alpha(PDGFRA) gene in childhood acute myeloid leukemia with t(8;21)(q22;q22) or inv(16)(p13q22).
Mechanisms of resistance to imatinib mesylate in gastrointestinal stromal tumors and activity of the PKC412 inhibitor against imatinib-resistant mutants.
CS-dependent response probability in an auditory masked-detection task: considerations based on models of Pavlovian conditioning.
Hiwatari M, Taki T, Tsuchida M, Hanada R, Hongo T, Sako M, Hayashi Y
Leukemia 2005 Mar;19(3):476-7
Leukemia 2005 Mar;19(3):476-7
Mechanisms of resistance to imatinib mesylate in gastrointestinal stromal tumors and activity of the PKC412 inhibitor against imatinib-resistant mutants.
Debiec-Rychter M, Cools J, Dumez H, Sciot R, Stul M, Mentens N, Vranckx H, Wasag B, Prenen H, Roesel J, Hagemeijer A, Van Oosterom A, Marynen P
Gastroenterology 2005 Feb;128(2):270-9
Gastroenterology 2005 Feb;128(2):270-9
CS-dependent response probability in an auditory masked-detection task: considerations based on models of Pavlovian conditioning.
Mason CR, Idrobo F, Early SJ, Abibi A, Zheng L, Harrison JM, Carney LH
The Quarterly journal of experimental psychology. B, Comparative and physiological psychology 2003 May;56(2):193-205
The Quarterly journal of experimental psychology. B, Comparative and physiological psychology 2003 May;56(2):193-205
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PDGFRA (arrow) using rabbit PDGFRA polyclonal antibody (Cat # PAB3940). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRA gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PDGFRA polyclonal antibody (Cat # PAB3940) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with PDGFRA polyclonal antibody (Cat # PAB3940) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
