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ImmunocytochemistryAntibody data
- Antibody Data
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- Validations
- Immunocytochemistry [2]
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- Product number
- ALX-210-099-C050 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-210-099-C050, RRID:AB_2052190
- Product name
- PDE5A polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Epitope-affinity purified.
- Reactivity
- Mouse, Bovine
- Host
- Rabbit
- Vial size
- 50 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references The soluble guanylate cyclase activator cinaciguat prevents cardiac dysfunction in a rat model of type-1 diabetes mellitus.
Mátyás C, Németh BT, Oláh A, Hidi L, Birtalan E, Kellermayer D, Ruppert M, Korkmaz-Icöz S, Kökény G, Horváth EM, Szabó G, Merkely B, Radovits T
Cardiovascular diabetology 2015 Oct 31;14:145
Cardiovascular diabetology 2015 Oct 31;14:145
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot using PAb to PDE5A (Prod. No. ALX-210-099) at 1:1'000 dilution.Lane 1) 100µg of PDE5A-deficient SKN-BE2 neuroblastoma lysate. Lane 2) 100µg lysate mixed with 5ng of purified recombinant bovine PDE5A (Prod. No. ALX-201-257). Lane 3) 3ng of partially purified recombinant bovine PDE5A. Signal was developed for 5 sec. using ECL-Plus reagent (Amersham).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot using PAb to PDE5A (Prod. No. ALX-210-099) at 1:1'000 dilution.Lane 1) 200µg of 15'000 x g supernatant of lysate from mouse brain. Lane 2) Mouse heart. Lane 3) Mouse lung. Lane 4) Mouse liver. Lane 5) 5ng of purified bovine PDE5.
