720030

dJ1112F19.1, ZNF797

Antibody data

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Validation data

Product number: 720030
Provider: Invitrogen Antibodies
Product name: Anti-SALL4 Polyclonal Antibody
Provider product page: Invitrogen Antibodies - 720030
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with Monkey, Rat, Mouse, Pig, Bovine, Rabbit and Dog. 720030 can be used for immunofluorescence analysis of Sall4 in human iPSCs and human ESC lines.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence was performed on fixed and permeabilized A-431cells for detection of Sall4 using Anti-Sall4 Rabbit Polyclonal Antibody (Product # 720030, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of Sall4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 594 Phalloidin (Product # A12381, 1:200). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Sall4. Panel e) represents control cells with no primary antibody to assess background.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Sall4 (red) in human ESCs WIRB3 and HES2. The cells were fixed with 300 uL 4% paraformaldehyde solution for 15 min at RT in the dark, permeabilized with 300 uL 0.25% TritonX-100 solution (diluted in PBS) for 5 min at RT, and blocked with 300 uL 10% BSA solution (diluted in PBS) for 30 min in a 37C incubator. Cells were stained with a Sall4 polyclonal antibody (Product # 720030) at a dilution of 1:250 (2 µg/mL) in 3% BSA solution (diluted in PBS) at 4C overnight, and then incubated with a secondary antibody (Red). Nuclei (blue) was stained with DAPI (Product # D1306). Images were taken on a Leica DM5500 microscope at 10X magnification. Data courtesy of Dr. Jianlong Wang's lab.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Sall4 (green) in Episomal Human iPSC cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Sall4 polyclonal antibody (Product # 720030), at a dilution of 2 µg/mL for one hour at room temperature, and then incubated with a goat anti-rabbit Superclonal IgG secondary antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were counterstained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific EVOS FL at 40X magnification.