LS-C815838

antibody from LSBio

Targeting: AFG3L2 SCA28, SPAX5

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C815838

Provider

LSBio

Product name

AFG3L2 Antibody LS-C815838

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

AFG3L2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 uL anti-AFG3L2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-AFG3L2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 89 kDa. Observed band size: 95 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

AFG3L2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 uL anti-AFG3L2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-AFG3L2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 89 kDa. Observed band size: 95 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

AFG3L2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 uL anti-AFG3L2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-AFG3L2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 89 kDa. Observed band size: 95 kDa.