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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF1926 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat Rad17 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and rat Rad17 in Western blots.
- Reactivity
- Human, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O75943- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human and Rat Rad17 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line and, NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Rad17 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
