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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX105364 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105364, RRID:AB_1949757
- Product name
- BRCC45 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references The deubiquitinating enzyme complex BRISC is required for proper mitotic spindle assembly in mammalian cells.
Yan K, Li L, Wang X, Hong R, Zhang Y, Yang H, Lin M, Zhang S, He Q, Zheng D, Tang J, Yin Y, Shao G
The Journal of cell biology 2015 Jul 20;210(2):209-24
The Journal of cell biology 2015 Jul 20;210(2):209-24
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BRCC45 antibody detects BRE protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract10 % SDS-PAGEBRCC45 antibody (GTX105364) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BRCC45 antibody detects BRE protein by Western blot analysis.A. 30 ug C8D30 whole cell lysate/extract10 % SDS-PAGEBRCC45 antibody (GTX105364) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and BRCC45 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody (GTX105364) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody (GTX105364) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and BRCC45 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody (GTX105364) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRE(GTX105364) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BRE antibody immunoprecipitates BRE protein in IP experiments. IP Sample: 1000 £gg 293T whole cell lysate/extract A. Control with 2.5 £gg of preimmune rabbit IgG B. Immunoprecipitation of BRE protein by 2.5 £gg of BRE antibody (GTX105364) 10% SDS-PAGE The immunoprecipitated BRE protein was detected by BRE antibody (GTX105364) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
