Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF3366 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Atrial Natriuretic Peptide/ANP Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Atrial Natriuretic Peptide/ANP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BNP, rhANF, and rhPTCH-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Aberrant pro-atrial natriuretic peptide/corin/natriuretic peptide receptor signaling is present in maternal vascular endothelium in preeclampsia.
Gu Y, Thompson D, Xu J, Lewis DF, Morgan JA, Cooper DB, McCathran CE, Wang Y
Pregnancy hypertension 2018 Jan;11:1-6
Pregnancy hypertension 2018 Jan;11:1-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Atrial Natriuretic Peptide/ANP by Simple WesternTM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for Atrial Natriuretic Peptide/ANP at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Atrial Natriuretic Peptide/ANP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3366) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Atrial Natriuretic Peptide/ANP by Western Blot. Western blot shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyoctyes (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Atrial Natriuretic Peptide/ANP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Atrial Natriuretic Peptide/ANP at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Atrial Natriuretic Peptide/ANP in Human Prostate. Atrial Natriuretic Peptide/ANP was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Atrial Natriuretic Peptide/ANP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3366) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.