PA5-59787

antibody from Invitrogen Antibodies

Targeting: FEM1B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-59787

Provider

Invitrogen Antibodies

Product name

F1A alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: NDRENYDIIK TYHYLYLAML ERFQDGDNIL EKEVLPPIHA YGNRTECRNP QELESIRQDR DALHMEGLIV RERILGADNI DVSHPIIY

Concentration

0.9 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockout of F1A alpha was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR1054853_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of F1A alpha was performed by loading 60 µg of A549 Cas9 (Lane 1) and A549 F1A alpha KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with F1A alpha Polyclonal Antibody (Product # PA5-59787, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076) with the iBright™ FL1500 (Product # A44115). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to F1A alpha. Uncharacterized band was observed in both the samples at ~110 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of F1A alpha in human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a F1A alpha Polyclonal Antibody (Product # PA5-59787).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of F1A alpha in human cell line A-431 using a F1A alpha Polyclonal Antibody (Product # PA5-59787) shows localization to nucleoplasm and cytosol.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of F1A alpha in human testis using a F1A alpha Polyclonal Antibody (Product # PA5-59787) shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.