Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-19955 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- F1A alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (A) and rat (B) liver tissue lysates using a F1A alpha polyclonal antibody (Product # PA5-19955) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha Polyclonal Antibody (Product # PA5-19955) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-F1A alpha Polyclonal Antibody (Product # PA5-19955) and a 70 kDa band corresponding to Protein fem-1 homolog B was observed across Mouse Testis, Mouse Heart and Rat Testis, but not in Mouse Ovary and Rat Ovary. Tissue extracts (30 µg lysate) of Mouse Testis (Lane 1), Mouse Heart (Lane 2), Mouse Brain (Lane 3), Mouse Ovary (Lane 4), Rat Testis (Lane 5) and Rat Ovary (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of mouse liver cells using a F1A alpha polyclonal antibody (Product # PA5-19955) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha Polyclonal Antibody (Product # PA5-19955) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha Polyclonal Antibody (Product # PA5-19955) at 20 µg/mL. Green: F1A alpha Blue: DAPI staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of F1Aα in mouse liver tissue with F1A alpha Polyclonal Antibody (Product # PA5-19955) at 5 µg/mL.