34-8800

Pawlicki P, Hejmej A, Milon A, Lustofin K, Płachno BJ, Tworzydlo W, Gorowska-Wojtowicz E, Pawlicka B, Kotula-Balak M, Bilinska B
Protoplasma 2019 Mar;256(2):393-408

Hypoxia-regulated delta-like 1 homologue enhances cancer cell stemness and tumorigenicity.

Kim Y, Lin Q, Zelterman D, Yun Z
Cancer research 2009 Dec 15;69(24):9271-80

A cellular study of human testis development.

Ostrer H, Huang HY, Masch RJ, Shapiro E
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation 2007;1(5):286-92

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of c-Kit/CD117 was performed using 70% confluent log phase SH-SY5Y cells treated with 10 uM retinoic acid for 72 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with c-Kit/CD117 Rabbit Polyclonal Antibody (Product # 34-8800) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical staining of breast cancer tissue using Rb anti-c-Kit (Product # 34-8800).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of c-Kit/CD117 showing staining in the cytoplasm and membrane of paraffin-embedded human testis tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a c-Kit/CD117 polyclonal antibody (Product # 34-8800) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of c-Kit / CD117 was done on K-562 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with c-Kit / CD117 Rabbit Polyclonal Antibody (348800, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Photomicrograph of the pulmonary sections of the different groups immunostained with C-KIT antibody. (A) The lung of G1 and (B) lung of G2 showing scarcely expression of C-KIT antibody within the pulmonary tissues. (C) Lung of G3 showing numerous C-KIT positive mast cells (arrows). (D) Lung of G4 showing decrease of C-KIT positive mast cells. (E) Lung of G5 showing marked decrease C-KIT positive mast cells, bar = 50 um. (F) Quantitative score of the number of C-KIT positive mast cells. Y= indicates significance in comparing with G1 (P < 0.001), * indicates significance in comparison with G3 (P < 0.001) and # indicates significance in comparison with G4 (P < 0.05).