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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3360 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3360, RRID:AB_1575619
- Product name
- KIT polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human KIT.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400 μl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Deletion of Trp-557 and Lys-558 in the juxtamembrane domain of the c-kit protooncogene is associated with metastatic behavior of gastrointestinal stromal tumors.
Alterations of the c-kit gene in testicular germ cell tumors.
Identification of Tyr900 in the kinase domain of c-Kit as a Src-dependent phosphorylation site mediating interaction with c-Crk.
Wardelmann E, Losen I, Hans V, Neidt I, Speidel N, Bierhoff E, Heinicke T, Pietsch T, Büttner R, Merkelbach-Bruse S
International journal of cancer 2003 Oct 10;106(6):887-95
International journal of cancer 2003 Oct 10;106(6):887-95
Alterations of the c-kit gene in testicular germ cell tumors.
Sakuma Y, Sakurai S, Oguni S, Hironaka M, Saito K
Cancer science 2003 Jun;94(6):486-91
Cancer science 2003 Jun;94(6):486-91
Identification of Tyr900 in the kinase domain of c-Kit as a Src-dependent phosphorylation site mediating interaction with c-Crk.
Lennartsson J, Wernstedt C, Engström U, Hellman U, Rönnstrand L
Experimental cell research 2003 Aug 1;288(1):110-8
Experimental cell research 2003 Aug 1;288(1):110-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of KIT polyclonal antibody (Cat # PAB3360) in mouse pancreas lysate. KIT (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of KIT polyclonal antibody (Cat # PAB3360) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with KIT polyclonal antibody (Cat # PAB3360) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H292 cells using KIT polyclonal antibody (Cat # PAB3360)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
