A01335-1

antibody from Boster Biological Technology

Targeting: KIT C-Kit, CD117, PBT, SCFR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

A01335-1

Provider

Boster Biological Technology

Product name

Anti-c-Kit/Kit Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for c-Kit/Kit detection. Tested with WB, IHC-P in Human.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Double sex and mab-3 related transcription factor 1 regulates differentiation and proliferation in dairy goat male germline stem cells.

Wei Y, Cai S, Ma F, Zhang Y, Zhou Z, Xu S, Zhang M, Peng S, Hua J
Journal of cellular physiology 2018 Mar;233(3):2537-2548

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Identification and Distribution of the Interstitial Cells of Cajal in the Abomasum of Goats.

Wang L, Liang Y, Chen Q, Ahmed N, Wang F, Hu B, Yang P
Cell transplantation 2018 Feb;27(2):335-344

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Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis.

Yang J, Chi C, Liu Z, Yang G, Shen ZJ, Yang XJ
Journal of cellular and molecular medicine 2015 Jul;19(7):1720-8

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Impact of the alterations in the interstitial cells of Cajal on intestinal motility in post-infection irritable bowel syndrome.

Yang B, Zhou XC, Lan C
Molecular medicine reports 2015 Apr;11(4):2735-40

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Wnt1 inhibits hydrogen peroxide-induced apoptosis in mouse cardiac stem cells.

Liu J, Wang Y, Du W, Liu W, Liu F, Zhang L, Zhang M, Hou M, Liu K, Zhang S, Yu B
PloS one 2013;8(3):e58883

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The inhibition of Endostar on the angiogenesis and growth of gastrointestinal stromal tumor xenograft.

Wang TB, Wei XQ, Lin WH, Shi HP, Dong WG
Clinical and experimental medicine 2012 Jun;12(2):89-95

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Embryonic hepatocyte transplantation for hepatic cirrhosis: efficacy and mechanism of action.

Bin WT, Ma LM, Xu Q, Shi XL
World journal of gastroenterology 2012 Jan 28;18(4):309-22

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An immunohistochemical study of S-100 protein in the intestinal tract of Chinese soft-shelled turtle, Pelodiscus sinensis.

Bao HJ, Liu Y, Qin JH, Xu CS, Hei NN, Jaber JR, Chen QS
Research in veterinary science 2011 Dec;91(3):e16-24

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Cancer stem-like cells can be isolated with drug selection in human ovarian cancer cell line SKOV3.

Ma L, Lai D, Liu T, Cheng W, Guo L
Acta biochimica et biophysica Sinica 2010 Sep;42(9):593-602

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The expression of Cajal cells at the obstruction site of congenital pelviureteric junction obstruction and quantitative image analysis.

Yang X, Zhang Y, Hu J
Journal of pediatric surgery 2009 Dec;44(12):2339-42

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Ginsenosides promote proliferation of chicken primordial germ cells via PKC-involved activation of NF-kappaB.

Ge C, Zhang C, Ye J, Tang X, Wu Y
Cell biology international 2007 Oct;31(10):1251-6

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Activation of protein kinases A and C promoted proliferation of chicken primordial germ cells.

Tang X, Zhang C
Animal reproduction science 2007 Oct;101(3-4):295-303

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Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.

Zhong XG, He S, Yin W, Deng JY, Cheng B
World journal of gastroenterology 2007 Jul 28;13(28):3886-91

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Stimulating effects of androgen on proliferation of cultured ovarian germ cells through androgenic and estrogenic actions in embryonic chickens.

Liu H, Zhang C, Tang X, Zeng W, Mi Y
Domestic animal endocrinology 2005 May;28(4):451-62

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Effects of follicle-stimulating hormone and androgen on proliferation of cultured testicular germ cells of embryonic chickens.

Mi Y, Zhang C, Xie M, Zeng W
General and comparative endocrinology 2004 Sep 15;138(3):237-46

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Effects of follicle-stimulating hormone and 17beta-estradiol on proliferation of chicken embryonic ovarian germ cells in culture.

Xie M, Zhang C, Zeng W, Mi Y
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2004 Dec;139(4):521-6

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of KIT using anti-KIT antibody (A01335-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIT antigen affinity purified polyclonal antibody (Catalog # A01335-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIT at approximately 145KD. The expected band size for KIT is at 110KD.

Additional image