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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-29974 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH7A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, mouse liver, rat liver.
- Concentration
- 0.98 mg/mL
Submitted references Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations.
Meierhofer D, Halbach M, Şen NE, Gispert S, Auburger G
Molecular & cellular proteomics : MCP 2016 May;15(5):1728-39
Molecular & cellular proteomics : MCP 2016 May;15(5):1728-39
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ALDH7A1 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ALDH7A1 Polyclonal Antibody (Product # PA5-29974) at a dilution of 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ALDH7A1 Polyclonal Antibody (Product # PA5-29974). Sample (30 µg of whole cell lysate). Lane A: Hela . 7.5% SDS PAGE. ALDH7A1 Polyclonal Antibody . ALDH7A1 Polyclonal Antibody (Product # PA5-29974) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ALDH7A1 Polyclonal Antibody (Product # PA5-29974). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. ALDH7A1 Polyclonal Antibody (Product # PA5-29974) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ALDH7A1 showing staining in the mitochondria and nucleus of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using an ALDH7A1 polyclonal antibody (Product # PA5-29974) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH7A1 Polyclonal Antibody [C1C3] detects ALDH7A1 protein at mitochondria and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37&#deg;C for 30 min. Green: ALDH7A1 protein stained by ALDH7A1 Polyclonal Antibody [C1C3] (Product # PA5-29974) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH7A1 Polyclonal Antibody detects ALDH7A1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ALDH7A1 Polyclonal Antibody (Product # PA5-29974) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
