H00001591-M07

antibody from Abnova Corporation

Targeting: CYP24A1 CP24, CYP24, P450-CC24

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001591-M07

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001591-M07, RRID:AB_581675

Product name

CYP24A1 monoclonal antibody (M07), clone 1F8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant CYP24A1.

Antigen sequence

LMLNTQVLGSSEDNFEDSSQFRPERWLQEKEKINP
FAHLPFGVGKRMCIGRRLAELQLHLALCWIVRKYD
IQATDNEPVEMLHSGTLVPSRELPIAFCQR


Isotype

IgG

Antibody clone number

1F8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CYP24A1 expression in transfected 293T cell line by CYP24A1 monoclonal antibody (M07), clone 1F8.Lane 1: CYP24A1 transfected lysate (Predicted MW: 11.11 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CYP24A1 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol