A700-004-T

antibody from Invitrogen Antibodies

Targeting: BRD4 CAP, HUNK1, HUNKI, MCAP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-004-T

Provider

Invitrogen Antibodies

Product name

BRD4 Recombinant Rabbit Monoclonal Antibody (BL-149-2H5)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BL-149-2H5

Vial size

20 µL

Concentration

0.10 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD4 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, HEK293T, Jurkat, A-549, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (Product # A700-004 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody (Product # A304-523A).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse BRD4 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (Product # A700-004 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody (Product # A304-523A).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD4 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-BRD4 recombinant monoclonal [[BL-149-2H5] (A700-004) and rabbit anti-BRD4 (A301-985A, BL5482). Secondary: ReliaBLOT® reagents (WB120).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD4 by WB of HeLa, 293T, Jurkat, MB231, HepG2, A549, SW620, and SK-MEL28 (SK) lysate. Antibody: Rabbit anti-BRD4 recombinant monoclonal [[BL-149-2H5] (A700-004). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD4 by immunocytochemistry. Sample: FFPE section of HeLa cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody (Product # A700-004 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD4 (orange) in formaldelyde-fixed HeLa cells by ICC-IF. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (A700-004). Secondary: DyLight® 550-conjugated goat anti-rabbit IgG (A120-201D3). Counterstain: Phalloidin Alexa Fluor® 488-conjugated (green).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD4 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody (Product # A700-004 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD4 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (A700-004). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (Product # A700-004 lot 3) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG (A120-201D5).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (A700-004).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (Product # A700-004 lot 4) used for IP at 20 µL/mg lysate. BRD4 was also immunoprecipitated by a previous lot of this antibody (A700-004 lot 3) and a second antibody against a different epitope of BRD4 (Product # A700-005). For blotting immunoprecipitated BRD4 (Product # A700-004) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.