RQ6828

antibody from NSJ Bioreagents

Targeting: BRD4 CAP, HUNK1, HUNKI, MCAP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6828

Provider

NSJ Bioreagents

Product name

BRD4 Antibody

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Bromodomain-containing protein 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) 293T, 2) Jurkat, 3) K562, 4) MCF7, 5) PC-3 and 6) HL60 cell lysate with Bromodomain-containing protein 4 antibody. Predicted molecular weight ~156 kDa (long form, can be observed at 200+ kDa) and ~81 kDa (short form).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human PC-3 cells with Bromodomain-containing protein 4 antibody.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-2 OS cells with Bromodomain-containing protein 4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bromodomain-containing protein 4 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of rat C6 cells with Bromodomain-containing protein 4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bromodomain-containing protein 4 antibody.