Explore
Validate
LearnNB300-514
antibody from Novus Biologicals
Targeting: IGF2R
CD222, CI-M6PR, CI-MPR, CIMPR, M6P-R, MPR1, MPR300, MPRI
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB300-514 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-514, RRID:AB_2139105
- Product name
- Mouse Monoclonal IGF-II R/IGF2R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Simian
- Host
- Mouse
- Antigen sequence
In the extracellular domain of Mann
ose 6 Phosphate Receptor (Cation in
dependent).- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Optogenetic Control of Synaptic AMPA Receptor Endocytosis Reveals Roles of LTD in Motor Learning.
Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models.
Rab9 GTPase regulates late endosome size and requires effector interaction for its stability.
Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor.
Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network.
Differential trafficking of live and dead Mycobacterium marinum organisms in macrophages.
Differential trafficking of live and dead Mycobacterium marinum organisms in macrophages.
Differential interaction with endocytic and exocytic pathways distinguish parasitophorous vacuoles of Coxiella burnetii and Chlamydia trachomatis.
Differential interaction with endocytic and exocytic pathways distinguish parasitophorous vacuoles of Coxiella burnetii and Chlamydia trachomatis.
Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor.
Kakegawa W, Katoh A, Narumi S, Miura E, Motohashi J, Takahashi A, Kohda K, Fukazawa Y, Yuzaki M, Matsuda S
Neuron 2018 Sep 5;99(5):985-998.e6
Neuron 2018 Sep 5;99(5):985-998.e6
Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models.
Takahashi K, Mashima H, Miura K, Maeda D, Goto A, Goto T, Sun-Wada GH, Wada Y, Ohnishi H
Scientific reports 2017 Jun 6;7(1):2817
Scientific reports 2017 Jun 6;7(1):2817
Rab9 GTPase regulates late endosome size and requires effector interaction for its stability.
Ganley IG, Carroll K, Bittova L, Pfeffer S
Molecular biology of the cell 2004 Dec;15(12):5420-30
Molecular biology of the cell 2004 Dec;15(12):5420-30
Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor.
Arighi CN, Hartnell LM, Aguilar RC, Haft CR, Bonifacino JS
The Journal of cell biology 2004 Apr;165(1):123-33
The Journal of cell biology 2004 Apr;165(1):123-33
Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network.
Valdez AC, Cabaniols JP, Brown MJ, Roche PA
Journal of cell science 1999 Mar;112 ( Pt 6):845-54
Journal of cell science 1999 Mar;112 ( Pt 6):845-54
Differential trafficking of live and dead Mycobacterium marinum organisms in macrophages.
Barker LP, George KM, Falkow S, Small PL
Infection and immunity 1997 Apr;65(4):1497-504
Infection and immunity 1997 Apr;65(4):1497-504
Differential trafficking of live and dead Mycobacterium marinum organisms in macrophages.
Barker LP, George KM, Falkow S, Small PL
Infection and immunity 1997 Apr;65(4):1497-504
Infection and immunity 1997 Apr;65(4):1497-504
Differential interaction with endocytic and exocytic pathways distinguish parasitophorous vacuoles of Coxiella burnetii and Chlamydia trachomatis.
Heinzen RA, Scidmore MA, Rockey DD, Hackstadt T
Infection and immunity 1996 Mar;64(3):796-809
Infection and immunity 1996 Mar;64(3):796-809
Differential interaction with endocytic and exocytic pathways distinguish parasitophorous vacuoles of Coxiella burnetii and Chlamydia trachomatis.
Heinzen RA, Scidmore MA, Rockey DD, Hackstadt T
Infection and immunity 1996 Mar;64(3):796-809
Infection and immunity 1996 Mar;64(3):796-809
Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor.
Dintzis SM, Velculescu VE, Pfeffer SR
The Journal of biological chemistry 1994 Apr 22;269(16):12159-66
The Journal of biological chemistry 1994 Apr 22;269(16):12159-66
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining of human testis using NB300-514 antibody at 1:100.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Mannose 6 Phosphate Receptor antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - An intracellular stain was performed on MCF7 cells with IGF-II R/IGF2R [2G11] Antibody NB300-514AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
