- PA3-850 - Invitrogen Antibodies IGF2R antibody

Submitted references Tumor protein D54 defines a new class of intracellular transport vesicles.

Larocque G, La-Borde PJ, Clarke NI, Carter NJ, Royle SJ
The Journal of cell biology 2020 Jan 6;219(1)

Chlamydia trachomatis CT229 Subverts Rab GTPase-Dependent CCV Trafficking Pathways to Promote Chlamydial Infection.

Faris R, Merling M, Andersen SE, Dooley CA, Hackstadt T, Weber MM
Cell reports 2019 Mar 19;26(12):3380-3390.e5

Arf6 controls retromer traffic and intracellular cholesterol distribution via a phosphoinositide-based mechanism.

Marquer C, Tian H, Yi J, Bastien J, Dall'Armi C, Yang-Klingler Y, Zhou B, Chan RB, Di Paolo G
Nature communications 2016 Jun 23;7:11919

Expression of insulin-like growth factor 2 receptor in corneal keratocytes during differentiation and in response to wound healing.

Bohnsack RN, Warejcka DJ, Wang L, Gillespie SR, Bernstein AM, Twining SS, Dahms NM
Investigative ophthalmology & visual science 2014 Oct 30;55(12):7697-708

Identification of novel murine- and human-specific RPGRIP1 splice variants with distinct expression profiles and subcellular localization.

Lu X, Ferreira PA
Investigative ophthalmology & visual science 2005 Jun;46(6):1882-90

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of CI-MPR was performed by loading various amounts of HeLa and Jurkat whole cell lysates per lane onto a 10% polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the Pierce G2 Fast Blotter (Product # 62288) and blocked with Pierce Fast Blocking Buffer (Product # 37575). The membrane was probed with a CI-MPR polyclonal antibody (Product # PA3-850) at a dilution of 1:2000 for at least 1 hour at room temperature, washed, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:75,000. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075), and the signal was captured using the Thermo Scientific myECL Imager (Product # 62236).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-IGF2R Polyclonal Antibody (Product # PA3-850) and a 274 kDa band corresponding to IGF2R was observed across cell lines and tissue tested along with uncharacterized (*) bands between 40-55 kDa. Whole Cell Extract (40 µg lysate) of Hep G2 (Lane 1), PANC-1 (Lane 2), Jurkat (Lane 3), THP-1 (Lane 4), HEL 92.1.7 (Lane 5), SW480 (Lane 6), A549 (Lane 7), Mouse Lung (Lane 8), Rat Lung (Lane 9) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CI-MPR (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CI-MPR polyclonal antibody (Product # PA3-850) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CI-MPR (green) showing staining in the cytoplasm and nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CI-MPR polyclonal antibody (Product # PA3-850) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CI-MPR (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CI-MPR polyclonal antibody (Product # PA3-850) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7. TPD54 co-reroutes dileucine motif-containing receptors only. (A) Representative widefield micrographs of cells coexpressing mCherry-FKBP-TPD54, dark MitoTrap, and the indicated CD8 construct. Rerouting was induced by 200 nM rapamycin. Cells were fixed, permeabilized, and stained for total CD8. (B) Representative widefield micrographs showing co-rerouting of endogenous CIMPR detected by immunofluorescence with rerouting of mCherry-FKBP-TPD54 to dark MitoTrap by addition of 200 nM rapamycin. (C) Pulse label and timed vesicle capture experiments. Cells expressing CD8-EAAALL were surface labeled with Alexa Fluor 488-conjugated anti-CD8 antibodies for 30 min, then incubated at 37degC for the indicated time (minutes), treated with 200 nM rapamycin for 5 min, and fixed. (D) Representative widefield micrographs from a pulse label and timed vesicle capture experiment. Inset, 5x zoom. Scale bars, 10 um, 1 um (insets). Time, hours and minutes.

PA3-850

Antibody data